Tissue Culture Propagation
Tissue Culture Propagation - Related Videos
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
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2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
12:52
Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
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Cited by 4 •
2014
Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
FAQs
Plant growth regulators determine which developmental pathway cultured cells follow. Under suitable conditions, they can stimulate cell division and callus formation, or promote organogenesis, the production of shoots and roots, and embryo development. This control allows researchers to direct undifferentiated tissue toward organized plant structures and ultimately recover plantlets from the selected explant.
Aseptic handling protects explants and cultures from unwanted microorganisms while they grow on nutrient media. Because the tissues remain in a controlled laboratory environment containing sugars, mineral salts, vitamins, and growth regulators, contamination could interfere with their development and prevent recovery of healthy plantlets. Maintaining sterility is therefore central to reliable propagation and preservation.
Propagation from a selected parent can produce genetically uniform clones, which helps maintain desirable genotypes across many plantlets. However, tissue culture conditions can also cause somaclonal variation, meaning genetic differences that arise among regenerated plants. This creates an important genetic distinction between using the method for faithful multiplication and using it to examine variation associated with culture.
The cultured explant is maintained on a nutrient medium while cells divide and may form callus, organs, or embryos. Regenerated shoots or plantlets then undergo rooting before acclimatization, a gradual preparation for transfer from laboratory conditions to soil. Each stage supports the transition from a small tissue sample to an established plant.
Researchers apply the method when they need rapid multiplication of valuable genotypes, preservation of germplasm, or production of disease-free plants. Starting with selected tissue makes it possible to expand important plant material under controlled conditions. These uses connect propagation with conservation and crop improvement efforts, particularly when maintaining a chosen genotype is a priority.
In genetics, cultures provide a way to compare the stability of regenerated plants with the selected parent and to investigate somaclonal variation produced under tissue culture conditions. Researchers can therefore use the same overall system both to preserve a genotype and to study how laboratory culture may be associated with genetic differences among regenerated plants.