Central Nucleus Amygdala
Central Nucleus Amygdala - Related Videos
10:48
How to Detect Amygdala Activity with Magnetoencephalography using Source Imaging
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Cited by 27 •
2013
This article describes how to record amygdala activity with magnetoencephalography (MEG). In addition this article will describe how to conduct trace fear conditioning without awareness, a task that activates the amygdala. It will cover 3 topics: 1) Designing a trace conditioning paradigm using backward masking to manipulate awareness. 2) Recording brain activity during the task using magnetoencephalography. 3) Using source imaging to recover signal from subcortical structures.
02:32
Generation of Tilted Amygdala Slices from a Mouse Brain
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2025
The video demonstrates the generation of tilted amygdala slices from a mouse brain. The brain is positioned at an angle on the vibratome specimen stage to obtain slices containing the amygdala and axons from the medial prefrontal cortex that form synaptic connections with the amygdala. The brain slices are maintained at the physiological temperature to preserve the tissue for further experiments.
04:18
Visualization and Mapping of Neuronal Pathways in an Amygdala Brain Slice
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2025
This video demonstrates a procedure for analyzing synaptic connectivity between medial prefrontal cortex (mPFC) and amygdala neurons using acute amygdala brain slices from mice. The mPFC neurons in these mice express channelrhodopsin fused to a fluorescent protein. The channelrhodopsins facilitate ion influx and action potential generation in the mPFC neurons upon light activation. Consequently, mPFC neurons release neurotransmitters that bind to postsynaptic amygdala neurons, triggering...
08:41
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
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Cited by 20 •
2016
Myocardial infarction (MI) is not only followed by impaired cardiac function but also by apoptosis in the amygdala, a brain region involved in the behavioral consequences of MI. This protocol describes how to induce MI, collect amygdala tissue and measure caspase-3 activity, a marker of apoptosis, therein.
02:05
Generating a Coronal Brain Slice Exposing the Amygdala Regions from a Mouse Brain
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2025
This video demonstrates the preparation of thin coronal brain slices from a murine brain, exposing the amygdala regions rich in synaptic connections from the medial prefrontal cortex. The slices are then recovered in oxygenated artificial cerebrospinal fluid to maintain neuronal viability.
FAQs
Their balance helps determine how strongly emotionally relevant signals are converted into downstream neural commands. Excitatory activity can promote output, while inhibitory activity can constrain or shape that signaling. In genetics research, examining gene expression in these neurons may reveal molecular influences on circuit state and associated defensive responses.
Its downstream connections allow neural activity associated with fear or stress to influence multiple response systems at once. Signals reaching the hypothalamus, brainstem, and autonomic nervous system can coordinate physiological changes with defensive behavior. This makes the region useful for studying how molecular variation affects integrated emotional and bodily responses.
Gene expression profiles can indicate which molecular programs are active in neurons of the central nucleus during emotionally relevant states. Comparing those patterns with circuit activity or behavioral responses may help connect cellular regulation to fear and stress processing. Such work can identify biological pathways associated with differences in emotional responsiveness.
Inherited variation refers to genetic differences that can contribute to how individuals respond to stress, whereas epigenetic regulation concerns changes in gene regulation that influence expression without changing the underlying genetic sequence. Examining both mechanisms helps researchers separate inherited influences from regulatory processes that may shape amygdala function and stress-related outcomes.
Researchers can ask how gene expression, inherited variation, and epigenetic regulation influence the activity of these neurons and their effects on emotional responses. They can also examine whether particular molecular patterns correspond with greater stress susceptibility. These questions connect cellular mechanisms with defensive behavior and the physiological responses associated with fear and stress.
Molecular findings from the central nucleus can clarify biological pathways that contribute to anxiety-related disorders. If gene-regulatory patterns are linked to altered fear, stress, or defensive responses, those pathways may provide potential targets for therapeutic development. The region therefore connects genetic research with both disease mechanisms and the search for intervention strategies.