Hepatic Stellate Cells
Hepatic Stellate Cells - Related Videos
06:40
Seven Steps to Stellate Cells
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Cited by 67 •
2011
Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.
07:06
Isolation and Primary Culture of Rat Hepatic Cells
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Cited by 137 •
2012
Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
02:52
Harvesting Hepatitis E Virus Particles from Human Liver Cancer Cells
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2026
Source: Meister, T. L., et al. A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks. J. Vis. Exp. (2020).This video demonstrates the process of isolating hepatitis E virus (HEV) particles from transfected human hepatocellular carcinoma cells. Extracellular viral particles are collected from filtered supernatants, while intracellular particles are released by subjecting the cells to repeated freeze-thaw cycles. The lysate is centrifuged, and the supernatant containing HEV...
09:35
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
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Cited by 2 •
2017
Here we describe a newly developed hepatitis B virus (HBV) reporter system to monitor the early stages of the HBV life cycle. This simplified in vitro system will aid in the screening of anti-HBV agents using a high-throughput strategy.
FAQs
Activation changes their functional state from vitamin A storage and structural maintenance toward a myofibroblast-like program. Injury, inflammatory cytokines, or infection can initiate this transition. Activated cells then produce extracellular matrix and reshape the hepatic microenvironment, linking local tissue damage with repair responses and, when persistent, disease-associated fibrogenesis.
Extracellular matrix production allows activated hepatic stellate cells to alter the physical and biochemical environment surrounding liver cells. This remodeling can support tissue repair, but it also contributes to fibrogenesis when activation continues. Studying this process helps researchers connect cellular responses to broader changes in liver structure during injury, inflammation, and infection.
Their behavior is shaped by communication with hepatocytes, Kupffer cells, endothelial cells, and lymphocytes. These interactions connect tissue repair with inflammatory and immune processes, rather than treating stellate cells as isolated structural cells. In infection research, this cellular network is important for examining how local liver responses influence pathogen control, inflammation, and disease progression.
Infection and inflammatory cytokines can provide signals that move hepatic stellate cells away from their healthy quiescent state. The resulting activation promotes extracellular matrix production and changes the hepatic microenvironment. This makes the cells relevant to studies of pathogen responses and to investigations of how persistent inflammation may connect immune activity with liver fibrogenesis.
They provide a cellular link between infection, tissue remodeling, and immune regulation. Research can examine how viral or parasitic pathogens, inflammatory cytokines, and neighboring immune cells affect stellate-cell activation. This perspective broadens infection studies beyond pathogen presence alone, helping investigators evaluate changes in the liver environment and potential host-directed strategies.
Because activated stellate cells contribute to extracellular matrix production and fibrogenesis, they offer a way to study mechanisms that reshape injured liver tissue. Investigators can use this biology to evaluate antifibrotic approaches and host-directed treatments that influence the liver response rather than targeting pathogens alone. Their interactions with immune and liver cells add therapeutic context.