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Seven Steps to Stellate Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Seven Steps to Stellate Cells

Seven Steps to Stellate Cells

DOI:
10.3791/2710-v

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06:40 min

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May 10, 2011

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, ,
1Immune Disease Institute, Program in Cellular and Molecular Medicine at Children’s Hospital, Department of Pathology,Harvard Medical School

Chapters

  • 00:05Title
  • 00:45In Situ Perfusion of Mouse Livers with Digestive Enzymes
  • 02:37In Vitro Digestion of Mouse Livers
  • 03:10Density Gradient Centrifugation
  • 05:29Results of Isolating Hepatic Stellate Cells
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.

Tags

Stellate CellsHepatic Stellate CellsLiver-resident CellsSpace Of DisseBone Marrow PrecursorsVitamin A StorageMyofibroblastsExtracellular MatrixLiver FibrosisPortal HypertensionAntigen Presenting CellsNKT CellsT LymphocytesIsolation Of Stellate CellsPerisinusoidal LocalizationDigestive EnzymesPronase ECollagenase PIn Vitro Enzymatic TreatmentVitamin A-storing Lipid DropletsCentrifugation

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