Hydrodynamic Shearing
Hydrodynamic Shearing - Related Videos
12:54
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
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Cited by 26 •
2014
In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.
10:13
A Microfluidic-based Hydrodynamic Trap for Single Particles
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Cited by 14 •
2011
In this article, we present a microfluidic-based method for particle confinement based on hydrodynamic flow. We demonstrate stable particle trapping at a fluid stagnation point using a feedback control mechanism, thereby enabling confinement and micromanipulation of arbitrary particles in an integrated microdevice.
11:12
Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves
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Cited by 5 •
2013
There has been renewed interest in developing polymer valves. Here, the objectives are to demonstrate the feasibility of modifying a commercial pulse duplicator to accommodate tri-leaflet geometries and to define a protocol to present polymer valve hydrodynamic data in comparison to native and prosthetic valve data collected under near-identical conditions.
04:53
A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome
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Cited by 5 •
2010
This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
FAQs
The key control is the velocity gradient, meaning that adjacent layers of liquid move at different speeds. This difference produces shear stress across biological material, while flow rate and channel geometry determine how that stress is distributed. Researchers can therefore adjust the physical conditions to promote deformation, detachment, fragmentation, or redistribution rather than applying an uncontrolled mechanical disturbance.
These variables determine the magnitude and distribution of the mechanical force experienced by the sample. Changing flow rate alters the applied force, viscosity changes how the liquid transmits stress, and channel geometry influences how fluid moves around or through the material. Controlling all three helps researchers create defined physical conditions for comparing biological responses.
Hydrodynamic shearing can act on cell aggregates, surface-associated microbes, and other biological material attached to or distributed within a flowing system. Depending on the selected conditions, the material may deform, detach from a surface, fragment into smaller units, or redistribute through the liquid. These distinct outcomes allow investigators to examine how physical forces influence biological organization.
A defined fluid force can challenge attachment without requiring direct mechanical contact with the sample. Observing whether cells or microbes remain attached, detach, fragment, or redistribute provides information about adhesion and the stability of biofilm-associated material. Comparing outcomes under different flow conditions can reveal how physical stress influences host-pathogen interfaces and microbial organization.
A general workflow begins by placing the biological material in a system where liquid can flow across a surface or through a channel. Researchers then select flow conditions based on the desired mechanical effect, expose the sample to that flow, and examine the resulting detachment, fragmentation, redistribution, or aggregate disruption. The processed material can then undergo downstream analysis.
Researchers should specify the flow rate, liquid viscosity, and channel geometry because these parameters determine the force applied to the sample. They should also identify whether the experiment is intended to disrupt aggregates, remove surface-associated microbes, or redistribute material. Defining these conditions in advance supports controlled comparisons between samples and clarifies how physical treatment affects the outcome.
Treatment can show how strongly surface-associated microbes or biofilm material withstand defined fluid forces. Measurements or observations of detachment, fragmentation, and redistribution help characterize biofilm stability and microbial attachment under controlled conditions. The resulting sample preparation may also support downstream analysis, allowing researchers to examine material that was previously aggregated or associated with a surface.
In this field, the technique connects physical forces with biological interactions. It can be used to study cell adhesion, disrupt cell aggregates, separate surface-associated microbes, and examine host-pathogen interactions under defined flow conditions. Applying controlled shearing also helps investigate immune-cell responses when biological material experiences mechanical environments that influence its organization or attachment.