Microtiter Plate Reader
Microtiter Plate Reader - Related Videos
12:23
Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
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Cited by 149 •
2011
In this protocol we present a method to measure Caenorhabditis elegans lifespan in 96 well microtiter plates.
05:16
A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic
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2025
This video demonstrates a microtiter plate-based method for determining the Minimum Inhibitory Concentration (MIC) of test antibiotics against bacteria. Upon adding a suspension of the bacterial pathogen Pseudomonas aeruginosa to a microtiter plate containing serial dilutions of a test antibiotic, and an inhibitor of the production of pyomelanin by the bacteria, the optical density of the wells is measured to compute the minimum inhibitory concentration of the antibiotic, and the impact of the...
12:39
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
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Cited by 29 •
2011
Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.
07:19
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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Cited by 105 •
2010
We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.
08:10
Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
0 Views •
Cited by 43 •
2015
C. elegans is an attractive model organism to study signal transduction pathways involved in oxidative stress resistance. Here we provide a protocol to measure oxidative stress resistance of C. elegans animals in liquid phase, using several oxidizing agents in 96 well plates.
FAQs
Signal selection must match the assay’s chemistry or biology. Absorbance records how much selected-wavelength light is measured after passing through a well, whereas fluorescence and luminescence detect emitted light generated by the assay. Choosing the appropriate mode allows chemical, enzymatic, or biological activity to be translated into a measurable signal and supports quantitative comparison among samples.
Wavelength selection matters because the instrument directs selected wavelengths into each well rather than using one universal optical setting. The appropriate wavelengths depend on the assay signal being measured, including absorbance, fluorescence, or luminescence. This assay-specific choice helps the reader detect the intended reaction output, which is essential when comparing antibody, antigen, cytokine, cellular, or microbial measurements.
Parallel plate format and automated reading address two practical needs: throughput and comparability. Samples positioned across many wells can be analyzed in the same measurement run, while automation improves consistency relative to repeated manual readings. In immunology and infection studies, this supports reproducible quantitative comparisons across diagnostic assays, treatment conditions, or host-pathogen experiments.
A typical workflow begins by arranging samples and assay reactions in the wells of a multiwell plate. The reader is then configured for the assay’s required optical signal and selected wavelengths. It measures each well in sequence or as part of an automated run, producing data that can be compared across samples to evaluate biochemical, cellular, or biological activity.
In ELISA-based studies, the reader measures the optical signal produced by reactions used to detect antibodies, antigens, or cytokines. Because many wells can be analyzed under the same assay framework, researchers can make quantitative comparisons among samples. This capability supports immunological investigations and diagnostic studies where the measured signal reflects the presence or activity of the target.
In infection research, plate-reader measurements can track microbial growth, cell viability, and immune-cell responses. The same high-throughput format also supports drug screening and host-pathogen investigations, allowing many experimental conditions to be compared efficiently. These readouts help connect treatment effects or infection-related changes with measurable biological activity across multiple samples.