Autologous Blood Perfusion
Autologous Blood Perfusion - Related Videos
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
07:02
Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice
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Cited by 28 •
2011
The autologous blood injection model of intracerebral hemorrhage in mice described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.
04:38
Inducing Massive Pontine Hemorrhage in a Rat Model by Autologous Blood Injection
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2025
Source: Tang, X. et. al., Massive Pontine Hemorrhage by Dual Injection of Autologous Blood. J. Vis. Exp. (2021)This protocol describes the controlled infusion of autologous blood into the pontine region of a rat's brain to model massive pontine hemorrhage. The purpose is to simulate a localized brain bleed by increasing localized pressure.
FAQs
A pump moves oxygenated blood through the tissue or organ’s vascular network, while pressure and flow help distribute oxygen and nutrients throughout the perfused region. This circulation also carries metabolic waste away from the tissue. Maintaining these transport functions allows the preparation to receive physiological support during preservation, assessment, or research use.
Autologous blood provides blood matched to the individual undergoing the procedure, reducing exposure to donor blood. This feature is especially relevant when clinicians or researchers need to maintain tissue support while limiting reliance on externally sourced blood. It also allows organ or tissue responses to be evaluated in a system using the patient’s own circulating material.
Monitoring tissue function provides information about how the tissue or organ responds while it receives oxygen and nutrients through its vascular network. In organ-preservation and transplantation research, these observations can help evaluate whether an organ remains viable under controlled perfusion conditions. The resulting assessment supplements preservation itself by providing functional information during the procedure.
Perfusion supplies an isolated organ with oxygenated blood while clinicians monitor its function, creating an opportunity to assess the organ under supported conditions. This approach can reveal whether the organ maintains functional responses during preservation or before transplantation-related decisions. It therefore connects circulation support with practical evaluation of organ viability rather than relying only on passive storage.
The procedure begins by directing the patient’s oxygenated blood into the tissue or isolated organ through a pump. Blood then travels through the vascular network, delivering oxygen and nutrients and removing metabolic waste. During circulation, clinicians can monitor tissue function. These coordinated steps provide both physiological support and a controlled setting for assessment or investigation.
Medical applications include organ preservation, ex vivo assessment, transplantation research, and selected surgical uses. In these settings, the technique can maintain an organ or tissue with circulating blood while its condition or responses are examined. Its value is greatest when investigators need a supported, controlled model rather than an assessment based solely on an organ outside circulation.
The method creates a controlled model in which blood moves through the tissue’s vascular network while oxygen delivery, nutrient supply, waste removal, and tissue function can be observed. Researchers can use this setting to study how vascular and tissue responses occur under supported perfusion conditions. Such observations add experimental context to organ preservation and transplantation investigations.