Biliary Anatomy

Biliary anatomy is the structural organization of the liver, gallbladder, and bile ducts that produces, stores, and transports bile for digestion and waste excretion. Hepatocytes secrete bile into canaliculi, which drain through intrahepatic ducts into the right and left hepatic ducts; these join as the common hepatic duct, while the cystic duct connects the gallbladder and the common bile duct carries bile toward the duodenum. Understanding this branching system helps clinicians interpret imaging, recognize obstruction, inflammation, gallstones, and anatomical variations, and plan procedures such as cholecystectomy, endoscopic interventions, and hepatobiliary surgery.

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Research

JoVE EoE - Rodent Models

Biliary System Resin Casting: A Technique for Analyzing 3D Architecture of Biliary System in Murine Model Using Radiopaque Resin Injection

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2025

This video describes the procedure for biliary system resin casting, for understanding 3D biliary architecture. This technique provides insights into the internal diameter and network connectivity of the biliary system, which could be used for liver regeneration analysis.

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

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Cited by 2 •

2017

A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.

Endoscopic Ultrasound-Guided Biliary Drainage: Endoscopic Ultrasound-Guided Hepaticogastrostomy in Malignant Biliary Obstruction

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Cited by 1 •

2022

Endoscopic Ultrasound-Guided Biliary Drainage (EUS-BD) is an alternative method of biliary decompression in malignant biliary obstruction. Here we describe the technique of EUS guided-Hepaticogastrostomy (EUS-HGS) in a case of unresectable malignant hilar biliary obstruction.

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

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Cited by 18 •

2015

To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.

Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion

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2025

This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.

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