Cerebral Region Collection
Cerebral Region Collection - Related Videos
07:06
Collection of Frozen Rodent Brain Regions for Downstream Analyses
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Cited by 20 •
2020
This procedure describes the collection of discrete frozen brain regions to obtain high-quality protein and RNA using inexpensive and commonly available tools.
11:01
Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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Cited by 3 •
2019
Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.
05:23
Construction and Application of Cerebral Functional Region-Based Cerebral Blood Flow Atlas Using Magnetic Resonance Imaging-Arterial Spin Labeling
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2024
This study integrated magnetic resonance imaging- arterial spin labeling images to derive cerebral blood flow (CBF) atlas for cerebral functional regions. Comparing typical healthy and chronic cerebral ischemia CBF atlases revealed significant differences in regional CBF distributions, enabling rapid, noninvasive assessments of functional CBF to assist in diagnosis and evaluate therapeutics.
13:50
Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat
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Cited by 107 •
2011
Surgical induction of ischemic brain damage in the rat is a widely used model for stroke research. Here we demonstrate the induction of focal cerebral ischemia by occlusion of the middle cerebral artery. Visualization of the resulting infarct by histological staining and magnetic resonance imaging is also shown.
08:50
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
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2025
In vivo, intercellular communications underpinning many central nervous system diseases, such as glioblastoma (GBM), are notoriously difficult to measure and characterize. Here, we describe procedures of cerebral open-flow microperfusion (cOFM) that can be employed to sample interstitial fluid components in a longitudinal animal model of GBM.
FAQs
Anatomical landmarks provide reference points for identifying the boundaries of a target brain area before dissection. Precise separation at these boundaries helps maintain the region’s tissue architecture and cellular composition, reducing uncertainty about which structures the sample represents. This anatomical orientation is important when investigators compare findings across defined regions or relate structural changes to neural function.
Preserving tissue architecture, cellular composition, and molecular contents allows one specimen to support several forms of analysis without losing biologically relevant information. Structural integrity supports imaging and histology, while retained molecular contents enable biochemical testing and molecular profiling. Careful preservation therefore improves the ability to connect microscopic or molecular findings with regional brain characteristics.
Consistent collection methods make samples more comparable because each specimen is assigned and separated according to the same anatomical logic. This reduces variation caused by differences in regional boundaries or handling and helps investigators distinguish biological differences from collection-related differences. In medical research, that comparability supports studies of brain development, pathology, biomarkers, and treatment responses.
The workflow begins by identifying the relevant anatomical landmarks, followed by precise dissection to separate the selected region from surrounding tissue. The collected material is handled in a way that preserves its architecture, cellular composition, and molecular contents. Samples can then be directed toward imaging, histology, biochemical testing, or molecular profiling, depending on the research question.
Collected regions can provide complementary structural, cellular, biochemical, and molecular information. Imaging and histology can examine organization and tissue features, whereas biochemical testing and molecular profiling can characterize region-specific molecular contents. Considering these results together helps researchers investigate how regional properties relate to neural function, disease-associated changes, or responses to treatment.
This approach is useful when a study needs to connect a particular brain area with development, pathology, neural function, or treatment response. It also supplies material for investigating region-specific biomarkers and mechanisms. By organizing samples according to defined anatomical areas, researchers can compare specimens systematically and examine whether disease-associated or treatment-related changes differ across regions.