Duplex Sonography
Duplex Sonography - Related Videos
11:42
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
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2012
Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.
04:10
Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
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2025
In this video, we demonstrate the procedure to determine the helicase activity of a target protein to unwind the biotin-labeled dsRNA substrate. The activity of the enzyme was identified by analyzing the electrophoretic mobility shift, followed by a chemiluminescence assay using chemiluminescent enzyme-conjugated streptavidin.
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
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2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
03:14
Spinal Sonography for Ultrasound-Guided Lumbar Neuraxial Anesthesia
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2025
The protocol described here provides the basics of spine sonoanatomy and a quick, straightforward method for performing ultrasound-guided neuraxial anesthesia. Additionally, two handheld devices that enhance portability are presented, one of which uses pattern recognition software to aid in epidural space localization.
07:31
Image Acquisition using Portable Sonography for Emergency Airway Management
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Cited by 1 •
2022
Point of care ultrasound (POCUS) is increasingly being utilized in airway management. Presented here are some clinical utilities of POCUS, including differentiating endotracheal and esophageal intubation, identification of the cricothyroid membrane in the event a surgical airway is required, and measuring anterior neck soft tissue to predict difficult airway management. ...
FAQs
The two data streams answer different questions. Grayscale imaging shows vessel or organ anatomy in two dimensions, while Doppler analysis interprets frequency shifts from moving blood to indicate flow direction and velocity. Viewing them together lets clinicians relate a structural finding to hemodynamic behavior, rather than assessing anatomy or circulation in isolation.
Doppler frequency shifts provide evidence about how blood is moving through the examined region. Their interpretation supplies flow direction and velocity, adding functional information to the structural image. This distinction matters when clinicians evaluate whether an anatomical abnormality is associated with altered circulation and when they monitor blood movement during a real-time examination.
Real-time imaging allows anatomy and circulation to be assessed as the examination proceeds, with structural and hemodynamic information available together. That immediate combination can support clinical evaluation of vascular abnormalities and circulation in organs or transplanted tissues. It also makes the technique useful for diagnosis, treatment planning, and follow-up rather than for structural imaging alone.
Duplex sonography can evaluate narrowing, obstruction, aneurysms, and thrombosis in blood vessels. Its value comes from showing the relevant anatomy while also assessing associated blood-flow behavior. This combination helps clinicians characterize vascular disease and supplies information that can contribute to diagnostic decisions, planning an intervention or other treatment, and evaluating changes over time.
The technique can assess circulation not only in blood vessels but also in organs and transplanted tissues. Doppler measurements provide information about blood movement, while grayscale imaging supplies the corresponding anatomical view. This broader use extends the method beyond isolated vessel assessment and supports clinical evaluation of tissue perfusion-related circulation without ionizing radiation.
Its usefulness follows from three combined features: noninvasive examination, real-time structural and flow assessment, and the absence of ionizing radiation. These characteristics allow clinicians to investigate vascular disease, obtain information relevant to treatment planning, and reassess circulation during follow-up. The method therefore supports repeated clinical evaluation when both anatomy and blood-flow behavior matter.