Human Islet Isolation
Human Islet Isolation - Related Videos
08:35
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
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Cited by 27 •
2009
Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
11:10
Isolation of Human Islets from Partially Pancreatectomized Patients
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Cited by 10 •
2011
The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
15:20
Murine Pancreatic Islet Isolation
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Cited by 119 •
2007
FAQs
Collagenase is introduced by perfusing the donated pancreas, allowing the enzyme to act throughout the tissue before mechanical dissociation. This combination helps release islets from surrounding exocrine tissue rather than relying on physical disruption alone. Controlled processing conditions are important because the procedure must separate the tissue sufficiently for subsequent purification and assessment.
Density-gradient centrifugation separates the released islets from other pancreatic material according to differences in density. This step enriches the islet fraction after collagenase treatment and mechanical dissociation, producing material that can be evaluated for purity. The resulting enrichment is important when researchers need preparations suitable for functional studies, transplantation investigations, or drug screening.
Three key assessments are purity, viability, and glucose-stimulated insulin secretion. Purity indicates how effectively islets were separated from exocrine tissue, while viability reflects the condition of the recovered cells. Glucose-stimulated insulin secretion tests whether the preparation retains a functional response relevant to regulated insulin production, helping researchers interpret experimental results more reliably.
The workflow begins with a donated human pancreas and perfusion with collagenase. Researchers then mechanically dissociate the tissue under controlled conditions, collect the released islets, and apply density-gradient centrifugation for purification. The preparation is subsequently assessed for purity, viability, and glucose-stimulated insulin secretion before it is selected for a particular study or application.
Isolated human islets provide material for transplantation studies, drug screening, and investigations of pancreatic biology. Because the preparation can be tested for beta-cell function and glucose-stimulated insulin secretion, it supports research into how insulin production is regulated. These studies can also examine diabetes-related dysfunction and evaluate approaches intended to restore controlled insulin production.
The procedure supplies human islet material for examining beta-cell performance and testing strategies aimed at restoring regulated insulin production. In medicine-focused research, investigators can use the isolated cells to study pancreatic function, model diabetes-related dysfunction, and assess transplantation approaches. Functional testing helps connect the quality of the preparation with its usefulness in these investigations.