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JoVE Journal
Biology

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Human Pancreatic Islet Isolation
 

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Article DOI: 10.3791/1343 08:36 min
May 26th, 2009

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Summary May 26th, 2009

Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method.

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