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Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
JoVE Journal
Biology
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JoVE Journal Biology
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
DOI:

08:35 min

May 26, 2009

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Chapters

  • 00:00Title
  • 00:32Introduction
  • 01:05Purification of Islet
  • 05:03Sampling and Culture
  • 07:08Representative Outcome: Evaluate Islet Purity and Continue
  • 07:25Conclusion

Summary

Automatic Translation

Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part II: purification and culture of human islets) using a modified automated method.

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