Lymph Vessel Cannulation
Lymph Vessel Cannulation - Related Videos
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
13:17
The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
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Cited by 48 •
2015
Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.
01:16
Lymphatic Vessels and Lymph Transport
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2024
Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells. This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular spaces.
08:12
Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels
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2023
This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.
06:25
Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs
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Cited by 6 •
2016
We present a surgical procedure to catheterize the intestinal lymph trunk in neonatal pigs to collect large quantities of lipid metabolism components from efferent lymph.
FAQs
The catheter must remain within the vessel lumen and be secured carefully. This positioning creates a stable route for lymph to enter or leave the vessel under controlled conditions. By limiting displacement and leakage, the technique helps preserve the sample and reduce disruption to lymphatic transport during measurement or substance administration.
Lymphatic vessels are accessed through precise microsurgery, so magnification helps the operator identify and manipulate the vessel accurately. Isolation separates the target vessel from surrounding structures before it is opened. These steps support accurate catheter placement while minimizing vessel damage and reducing the loss of lymph around the access site.
Controlled flow allows investigators to collect lymph directly or administer substances through a defined access point. This makes it possible to examine lymph composition, assess lymphatic transport, and study how material moves through the lymphatic system. The resulting measurements can connect lymph flow with fluid balance, immunity, inflammation, or disease progression.
The core workflow includes working under magnification, isolating the selected lymphatic vessel, opening the vessel, and placing a fine catheter inside its lumen. The catheter is then secured to maintain access and limit leakage. Careful completion of these stages supports controlled collection or administration while minimizing damage to the vessel.
Researchers would use Lymph Vessel Cannulation when they need direct access to lymph or controlled delivery into a lymphatic vessel. The method is useful for analyzing lymph contents, measuring transport, investigating immune-cell trafficking, or evaluating therapies that target lymphatic function. Its value comes from connecting experimental interventions with measurable lymphatic responses.
Direct lymphatic access helps researchers examine the system's involvement in fluid balance, immunity, inflammation, and disease progression. It can also support evaluation of treatments designed to alter lymphatic function. By enabling collection and administration under controlled conditions, the technique provides experimental information about lymphatic responses that may be difficult to assess indirectly.