Organ Ex Vivo Imaging
Organ Ex Vivo Imaging - Related Videos
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
11:51
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Cited by 9 •
2009
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
07:15
Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
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Cited by 1 •
2025
Optical clearing techniques, such as absorbing molecules solutions, reduce light scattering temporarily to enhance biological imaging and offer biocompatible, reversible in vivo imaging strategies for deeply embedded organs. This protocol presents detailed experimental procedures as well as advanced image processing to achieve high-quality, dynamic imaging for real-time dynamics in living animals.
14:04
Viral Nanoparticles for In vivo Tumor Imaging
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Cited by 45 •
2012
Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.
04:09
An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions
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2025
In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue viability.
FAQs
Maintaining tissue in a controlled environment with oxygenated media or perfusion helps preserve the organ’s structure and function after removal. This support allows observations to reflect biological features rather than changes caused solely by inadequate maintenance. The choice is therefore central when interpreting cellular activity or disease-related alterations outside the body.
Organ ex vivo imaging can connect findings at the whole-organ level with cellular processes. Researchers can examine macroscopic tissue changes while also capturing cellular activity in the same removed specimen. This combined view helps relate visible pathology to underlying biology, making the approach useful for investigating disease-related changes and treatment effects rather than treating anatomy and cell behavior as separate observations.
Optical, fluorescent, and other imaging methods provide complementary ways to capture biological features in the removed organ. Optical approaches can document tissue appearance, whereas fluorescent approaches can capture biological features through fluorescence-based visualization. Applying these modalities supports analysis across anatomical and cellular scales, helping researchers relate tissue organization to activity or disease-related changes.
After surgical removal, the organ is placed in a controlled imaging environment and may be maintained with oxygenated media or perfusion. Researchers then apply optical, fluorescent, or other imaging methods to capture relevant features, followed by analysis of anatomy, cellular activity, or disease-related changes. This sequence supports examination while the specimen remains experimentally supported outside the body.
Medical researchers use Organ ex vivo imaging to study pathology, evaluate treatment response, investigate organ preservation, and examine transplantation-related questions. Because the organ can be analyzed outside the body, the method supports direct study of tissue changes and cellular activity in a controlled setting. These applications make it relevant to both experimental investigation and clinically oriented research.
The method allows researchers to examine an organ while assessing how well its structure and function are maintained outside the body. Imaging can reveal anatomical and cellular features relevant to preservation studies, while the same information can inform transplantation research. It therefore provides a way to investigate organ condition before connecting experimental findings with clinical concerns.