Oxygen Glucose Deprivation
Oxygen Glucose Deprivation - Related Videos
02:34
Impact of Oxygen-Glucose Deprivation and Reoxygenation on Cell-Cell Interactions
0 Views •
2025
Source: Alluri, H., et.al. Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction. J. Vis. Exp. (2015).The video demonstrates the impact of oxygen-glucose deprivation and subsequent reoxygenation on rat brain microvascular endothelial cells (RBMECs) by simulating hypoxic and nutrient-deprived conditions. These conditions result in ATP depletion, increased intracellular calcium, and the generation of reactive...
08:56
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
0 Views •
Cited by 46 •
2015
Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
02:33
Assessing Atmospheric Pressure Plasma Treatment in Glucose-Deprived Neuroblastoma Cells
0 Views •
2025
Source: Yan, X., et. al., New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells. J. Vis. Exp. (2017)The video demonstrates the neuroprotective activity of low-dose atmospheric pressure plasma treatment in glucose-deprived neuroblastoma cells by modulating oxidative stress and enhancing cell viability.
05:52
In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement
0 Views •
2025
This video demonstrates an in vitro assay to indirectly measure intracellular glucose uptake. The assay uses a fluorescently-labeled glucose analog to establish a correlation between extracellular glucose depletion and intracellular glucose uptake.
02:23
Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice
0 Views •
2023
This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.
FAQs
Oxygen deprivation directly compromises mitochondrial respiration, while the simultaneous loss of glucose limits the substrate available for ATP production. As cellular energy falls, ion balance and membrane function become disrupted, and survival pathways are affected. These linked changes allow investigators to connect metabolic failure with downstream cellular injury under ischemic conditions.
Limiting both resources creates a combined metabolic and respiratory challenge rather than isolating either stress. Oxygen shortage interferes with mitochondrial respiration, while glucose depletion restricts ATP production. Studying them together therefore helps researchers examine how impaired energy generation contributes to disrupted ion balance, membrane function, and cell survival during ischemic injury.
Reoxygenation and nutrient restoration recreate reperfusion after the deprivation phase. This transition lets investigators examine not only damage accumulated during OGD, but also cellular recovery and responses when oxygen and metabolic support return. Including this phase is therefore useful for studying protective treatments and mechanisms relevant to interrupted and restored blood flow.
In medicine, OGD supports investigation of ischemic stroke, heart injury, brain injury, and neurovascular responses. Its controlled deprivation and restoration phases help researchers connect changes in cellular energy availability with injury mechanisms and recovery. The model also provides a framework for evaluating protective treatments in disease contexts where blood flow is interrupted.
A basic OGD workflow restricts oxygen and glucose availability to cells or tissues for an experimental period, then may restore oxygen and nutrients to represent reperfusion. This sequence allows researchers to examine injury during deprivation and recovery after restoration within the same experimental framework, supporting comparisons between ischemic stress and subsequent cellular responses.
OGD outcomes can include evidence of impaired ATP-related energy supply, disrupted ion balance, altered membrane function, affected cellular survival pathways, and changes in recovery after restoration. These readouts help determine how ischemic injury develops and whether a protective treatment improves cellular responses during deprivation, reoxygenation, or both.