Anti-fade Mounting Medium
Anti-fade Mounting Medium - Related Videos
10:13
Method for Whole Mount Antibody Staining in Chick
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Cited by 3 •
2009
This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.
08:42
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
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Cited by 13 •
2014
Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.
07:50
A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training
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Cited by 21 •
2017
This protocol describes a novel and efficient method to quickly initiate operant responding for ethanol in rats that, contrary to standard methods, does not require water deprivation or saccharin/sucrose fading to initiate responding.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
13:10
Antibody Staining in C. Elegans Using "Freeze-Cracking"
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Cited by 34 •
2013
"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.
FAQs
Its protective effect addresses oxygen-dependent photochemical reactions that degrade fluorophores during illumination. By limiting this degradation, the medium helps fluorescent labels retain detectable signal for longer periods under the microscope. This is especially important when imaging requires repeated observation, documentation, or comparison of labeled structures within the same specimen.
Beyond protecting fluorophores, the medium provides a stable optical environment between the specimen and coverslip. This stability supports more consistent transmission of fluorescent information during imaging and reduces variation associated with the mounted preparation. As a result, images of neural structures can be documented more reliably across observations.
More persistent fluorescence gives investigators a better opportunity to capture images before signal loss substantially affects the specimen’s appearance. Maintaining signal consistency supports comparisons among labeled regions and improves confidence when documenting fluorescence intensity or distribution. In neuroscience, this can strengthen analysis of neural organization marked by fluorescent labels.
The medium is relevant to fixed brain tissue and cultured cells prepared for fluorescence microscopy. Within these specimens, it can support imaging of labeled neurons, axons, synaptic markers, and other fluorescently identified structures. Its use therefore spans both tissue-level organization and cellular preparations used to examine neural features.
It is particularly useful when fluorescently labeled specimens must be examined, recorded, or quantitatively compared under microscope illumination. Fixed brain tissue and cultured neural cells may contain signals that need careful visualization across structures such as axons or synaptic markers. Preserving those signals helps produce more dependable images for documenting neural organization.
The approach can extend the persistence of fluorescent signals and improve image consistency in mounted specimens. Those outcomes support clearer visualization and more reliable documentation of labeled neural structures, including neurons, axons, and synaptic markers. They also provide a stronger basis for quantitative analysis of how fluorescently identified features are arranged in tissue or cultured cells.