Ex Vivo Neuronal Preparation
Ex Vivo Neuronal Preparation - Related Videos
10:31
In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
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Cited by 3 •
2020
Integration of diverse synaptic inputs to neurons is best measured in a preparation that preserves all pre-synaptic nuclei for natural timing and circuit plasticity, but brain slices typically sever many connections. We developed a modified brain slice to mimic in vivo circuit activity while maintaining in vitro experimentation capability.
11:06
In vivo Neuronal Calcium Imaging in C. elegans
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Cited by 20 •
2013
With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.
11:58
In-vivo and Ex-vivo Calcium Imaging of an Olfactory Circuit Neuron in the Third-Instar Drosophila melanogaster Larva
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2025
We present calcium imaging protocols for a Drosophila larval olfactory neuron, using a topical tissue adhesive for immobilization. This method enhances stability, facilitating reliable in-vivo and ex-vivo experiments. Custom R scripts analyze calcium signals, providing an efficient platform for detailed neurophysiological research.
09:06
Preparation of Neuronal Co-cultures with Single Cell Precision
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Cited by 6 •
2014
Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs.
18:02
Preparation of Dissociated Mouse Cortical Neuron Cultures
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Cited by 146 •
2007
This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.
FAQs
Oxygenated physiological solutions help maintain the ions, nutrients, and dissolved oxygen needed for viable neural tissue. By preserving these basic conditions, the preparation can retain electrical activity and synaptic function long enough for controlled experiments. This environment allows researchers to examine neuronal communication directly while manipulating selected experimental variables in the laboratory.
Isolating a defined neural circuit reduces the complexity of the experimental system and helps connect an observed response to particular neurons or connections. Researchers can then examine synaptic transmission under controlled conditions rather than interpreting activity across the entire organism. This focused approach supports analysis of cellular mechanisms that may contribute to behavior or disease.
Electrophysiological recordings measure neuronal electrical activity and can be used to examine how neurons communicate through synaptic transmission. Because the tissue remains accessible outside the organism, researchers can observe activity while applying controlled experimental manipulations. These measurements help link cellular electrical responses with the function of a preserved neural circuit.
The basic workflow begins by removing neurons or neural tissue from the organism and transferring the material to controlled laboratory conditions. Researchers maintain the tissue in an oxygenated physiological solution containing essential ions and nutrients, then select an analysis such as electrophysiological recording, calcium or fluorescence imaging, or pharmacological manipulation. The sequence preserves function while enabling direct measurement.
The choice depends on the information required from the preparation. Electrophysiological recording is suited to studying neuronal electrical activity and synaptic transmission, whereas calcium or fluorescence imaging provides an imaging-based way to analyze activity in the maintained tissue. Pharmacological manipulation can be added when the experiment focuses on how drugs alter neuronal or network activity.
These preparations are used to investigate how neurons communicate, how drugs modify network activity, and how cellular mechanisms relate to behavior or disease. Their value comes from combining preserved neural function with experimental control. Researchers can therefore study defined circuits, measure responses directly, and examine drug or cellular effects without losing access to the tissue during the experiment.