/
/
In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:55Brain Removal with Intact Auditory Nerve Root
- 02:56Brain Tissue Blocking and Mounting
- 04:06Wedge Slice Generation
- 06:16Electrophysiology Setup and Recording
- 07:51Results: Representative Wedge Slice Preparation and Stimulation
- 09:44Conclusion
Integration of diverse synaptic inputs to neurons is best measured in a preparation that preserves all pre-synaptic nuclei for natural timing and circuit plasticity, but brain slices typically sever many connections. We developed a modified brain slice to mimic in vivo circuit activity while maintaining in vitro experimentation capability.
