Gfp-labeled Microglia
Gfp-labeled Microglia - Related Videos
07:25
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
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Cited by 5 •
2013
A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.
04:55
CRC Organoid Cell Labeling: A Method to Generate GFP Lentivirus-transduced Colorectal Cancer Organoid Cells
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2023
This video describes the technique of generating CRC organoids transduced by GFP lentiviral particles for enabling fluorescent imaging. These GFP labeled organoids, when placed in animal models, help to study tumor invasion and metastasis.
11:28
Culturing Microglia from the Neonatal and Adult Central Nervous System
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Cited by 35 •
2013
We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.
09:12
Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
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Cited by 17 •
2014
One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam).
13:36
Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
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Cited by 37 •
2012
Microglia are resident macrophages that provide the first line of defense and immune surveillance of the central nervous system. MicroRNAs are regulatory molecules that play an important role in many physiological processes including activation and differentiation of macrophages. In this article, we describe the method for measurement of microRNAs in microglia.
FAQs
GFP expression produces a fluorescent signal that can be detected under suitable excitation. Researchers can then visualize labeled cells within nervous tissue and examine changes in morphology, position, activation, and interactions with neurons or damaged regions. This converts microglial responses into image-based observations and supports monitoring during live-cell imaging.
Linking GFP expression to microglia-specific genetic activity helps associate the observed fluorescence with the intended immune cell population. This improves the interpretability of images because researchers can examine microglial structure, movement, and tissue interactions without treating fluorescence as an undifferentiated signal from the entire nervous system.
The method supports analysis of several cellular features rather than fluorescence alone. Investigators can assess microglial morphology, migration, activation, and interactions with neurons or damaged tissue. Considering these features together helps characterize how microglia respond to their local environment in nervous tissue and how those responses change across experimental models.
Visualization requires fluorescence imaging performed with suitable excitation for the GFP signal. Once the labeled cells are illuminated under those conditions, their distribution and cellular features can be examined in nervous tissue. The same imaging approach can support live-cell observation, allowing researchers to monitor microglial responses while the cells remain in the tissue model.
Researchers can apply this labeling strategy when they need to examine microglial responses in models of neurodevelopment, injury, inflammation, or neurodegenerative disease. It is especially useful when the study focuses on how these cells change their morphology, migrate, become activated, or interact with neurons and damaged tissue.
GFP-labeled microglia provide a way to track immune-cell behavior in nervous tissue during biologically important conditions. In injury, inflammation, and neurodegenerative disease models, imaging can reveal changes in microglial location, form, activation, and interactions with affected tissue. These observations help connect cellular responses with the surrounding neurological context.