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JoVE Journal
Immunology and Infection

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Culturing Microglia from the Neonatal and Adult Central Nervous System
 

Culturing Microglia from the Neonatal and Adult Central Nervous System

Article DOI: 10.3791/50647
August 9th, 2013

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Summary August 9th, 2013

We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

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