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Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

DOI:
10.3791/4097-v

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13:36 min

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July 23, 2012

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, , ,
1Center for Neurologic Diseases, Brigham and Women’s Hospital,Harvard Medical School

Chapters

  • 00:05Title
  • 01:34Isolation of Microglia from Normal CNS
  • 03:04Control of Purity of Microglia by Flow Cytometry
  • 04:27Isolation of Microglia and Peripheral Macrophages from Inflamed CNS in Mouse Model of Neuroinflamation
  • 05:43Isolation of RNA
  • 07:43Normalization and Data Analysis
  • 10:13Results: Analysis of miR-124 Expression in Microglia and Macrophages
  • 13:14Conclusion

Summary

Automatic Translation

Automatic Translation

Microglia are resident macrophages that provide the first line of defense and immune surveillance of the central nervous system. MicroRNAs are regulatory molecules that play an important role in many physiological processes including activation and differentiation of macrophages. In this article, we describe the method for measurement of microRNAs in microglia.

Tags

MicroRNAsMicrogliaReal-time PCRNeuroinflammationCNSCD11bCD45Activation MarkersPathological EventsPhenotypeFunctionsMiRNAsCell GrowthDifferentiationInflammationInnate Immune CellsMacrophagesMRNAExpression Analysis

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