Nmda Infusion
Nmda Infusion - Related Videos
07:11
Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity
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2025
Here, we describe the use of a genetically directed alkaline phosphatase (AP) labeling method to investigate the anatomical features and phenotypes of mouse retinas under NMDA-induced excitotoxicity. In conjunction with several retinal ganglion cell (RGC) subtype-specific reporter lines, we uncover novel phenotypes in dying RGCs throughout the degenerative process.
06:42
Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique
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Cited by 5 •
2010
In this video, we demonstrate how to apply a conductance into a dopaminergic neuron recorded in the whole cell configuration in rat brain slices. This technique is called the dynamic clamp.
08:18
A Model of Chronic Nutrient Infusion in the Rat
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Cited by 1 •
2013
A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.
10:19
Neurodegeneration in an Animal Model of Chronic Amyloid-beta Oligomer Infusion Is Counteracted by Antibody Treatment Infused with Osmotic Pumps
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Cited by 11 •
2016
To study the effects of Aβo in vivo, we developed a model based on repeated hippocampal infusions of soluble Aβo coupled with continuous infusion of Aβo antibody (6E10) in the hippocampus using osmotic pumps to counteract the neurotoxic effect of Aβo.
04:21
Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump
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2025
Source: Léa Rubira1, Hélène Sturer1, Louise Combette1, Sandra Santini1, Stéphane Renaud1, Cyril Fersing1,21Nuclear medicine department, Institut régional du Cancer de Montpellier (ICM), Univ Montpellier, 2IBMM, Univ Montpellier, CNRS, ENSCMThis video demonstrates the safe, controlled infusion of a radiopharmaceutical using a peristaltic pump with a dual-line cassette system. The setup includes connecting saline and infusion lines, positioning the shielded vial, and initiating simultaneous...
FAQs
The key change is not simply receptor engagement, but the resulting ionic load. NMDA receptor channels admit sodium and calcium, which depolarize the neuronal membrane. If activation persists, calcium-dependent stress can progress toward excitotoxicity and neuronal injury. Measuring responses under controlled exposure helps connect receptor activity with cellular damage.
Calcium entry provides a mechanistic link between excitatory signaling and neuronal vulnerability. Receptor-driven activity can be examined in relation to synaptic plasticity, whereas sustained activation is associated with excitotoxic stress. NMDA infusion experiments can therefore compare how neurons or experimental conditions respond to defined excitatory challenges and identify patterns associated with injury.
Controlled exposure provides a defined challenge against which neuronal responses can be examined. Investigators can assess whether the resulting signaling is associated with membrane depolarization, plasticity-related activity, seizure-related activity, or excitotoxic injury. This makes the approach useful for studying why some experimental conditions show greater neuronal damage or vulnerability than others.
A useful experiment must specify the exposure conditions and interpret responses in relation to the intended model. Researchers can examine receptor-mediated excitation, calcium and sodium entry, membrane depolarization, and signs of excitotoxic stress or injury. Keeping administration controlled helps distinguish effects of the experimental challenge from broader differences in the preparation.
The choice depends on the scientific question. NMDA infusion can support models of synaptic plasticity, seizure-related activity, neurodegeneration, and excitotoxicity. These applications allow researchers to examine receptor function and neuronal vulnerability in different contexts, rather than treating excitatory signaling as a single outcome. The observations can also inform interpretation of disease mechanisms.
In neuroscience, these models connect cellular receptor activity with larger questions about learning, memory, and disease. They also provide a framework for testing potential neuroprotective strategies by examining whether experimental conditions alter vulnerability to excitotoxic stress. Their value lies in linking defined NMDA-driven signaling to measurable neuronal outcomes relevant to both normal function and injury.