JoVE Encyclopedia of Experiments
Biological Techniques
0 views • 4:21 min • October 30th, 2025
Begin with a peristaltic infusion system assembled for radiopharmaceutical delivery.
The pump is loaded with an infusion cassette.
The cassette’s line A is connected to a saline bag and line B to a saline-purged spinal needle.
Connect the pump’s output tubing to the patient’s catheter.
Next, position a lead-shielded barrier in front of the pump to reduce radiation exposure to the operator.
Place a lead container holding the radiopharmaceutical vial on the barrier’s tray and remove its cap.
Disinfect the vial septum. Insert a venting filter, ensuring its tip remains above the liquid to prevent vacuum formation.
Then, insert the spinal needle from line B into the septum, directing the tip to the bottom of the vial.
Start the infusion. The pump draws saline through line A and radiopharmaceutical through line B and delivers them to the patient in a controlled manner.
To begin, insert the infusion set spike into the inlet of the 250 milliliter sodium chloride 0.9 percent bag. Apply gentle pressure to fill the infusion chamber three-quarters full.
Turn the perfusion set cassette upside down, declamp the infusion set, and slightly unscrew the flow regulator. As soon as the first drops of saline ent
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