Pulsatile Lh Measurement
Pulsatile Lh Measurement - Related Videos
10:02
Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
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Cited by 12 •
2011
The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
07:44
Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
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Cited by 1 •
2023
This method describes the encapsulation of the rabies antigen into biodegradable polymeric microparticles with structural and material properties that enable pulsatile release after a predetermined delay. Enzyme-linked immunosorbent assay (ELISA) assessment of the antigen retrieved from the particle core confirms the presence of intact trimeric rabies virus glycoprotein through particle fabrication.
08:15
Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice
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Cited by 18 •
2012
Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.
05:58
Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion
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Cited by 27 •
2018
Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors.
FAQs
Pulse intervals and amplitudes provide separate measures of episodic reproductive endocrine activity. Intervals describe the timing between LH rises, whereas amplitudes describe their magnitude. Because hypothalamic gonadotropin-releasing hormone activity drives pituitary LH release, examining both features helps researchers relate neural signaling patterns to downstream pituitary and gonadal function rather than relying on a single hormone value.
A single LH concentration provides a limited snapshot and cannot characterize the sequence of rises that defines episodic release. Serial measurements reveal when pulses occur, how far apart they are, and how large they become. This time-resolved information supports comparisons of hypothalamic-pituitary-gonadal axis function across reproductive states, developmental stages, or experimental conditions.
Pulsatile LH patterns provide an indirect readout of hypothalamic gonadotropin-releasing hormone activity because hypothalamic signaling drives episodic pituitary secretion. Researchers can therefore use changes in LH timing and magnitude to examine how neural regulation corresponds with pituitary output and gonadal function. This connection makes the measurement useful for neuroscience studies of reproductive endocrine control.
Researchers compare the timing, intervals, and amplitudes of LH rises before and after a physiological or experimental intervention. A changed pattern may indicate altered regulation within the hypothalamic-pituitary-gonadal axis, although the measurement specifically describes circulating LH dynamics. Its value lies in linking an intervention with coordinated changes in neural, pituitary, and gonadal function.
The workflow begins with repeated blood collection over time rather than a single sample. Each specimen is analyzed with a hormone assay, and the resulting LH concentrations are arranged chronologically. Investigators then identify rises, estimate intervals between pulses, and assess pulse amplitudes. These measurements create a profile that can be compared across subjects, conditions, or reproductive stages.
This approach is used when researchers need to examine dynamic hypothalamic-pituitary-gonadal axis function during puberty, menstrual cycling, fertility, aging, or reproductive disorders. It also supports studies of physiological and experimental interventions. By connecting serial LH patterns with neural regulation and gonadal activity, the method helps explain how reproductive endocrine signaling changes across these contexts.