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Chapters
- 00:05Title
- 01:13Ultrasound and Pressure System Overview and Set-up
- 03:23Mouse Preparation
- 04:55Ventricular Pressure Measurement with Ultrasound
- 07:10Pulsatile Pressure and Heart-rate Measurements
- 07:51Conclusion
Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.
Tags
Left Ventricular PressureLate Embryonic MiceNeonatal MiceBlood PressureVertebrate AnimalsMouse DevelopmentSystolic Left Ventricular PressureInvasive Pressure MeasurementsImaging MethodsMean Arterial PressurePostnatal DaySolid-state CathetersDeveloping Mouse ArteriesCustom Pulled Plastic CathetersBlood Pressure IncreaseSmooth Muscle Cell PhenotypeMechanotransduction EventsExtracellular Matrix Proteins
