Stepping Parameter Assessment

Stepping parameter assessment is a behavioral analysis method that quantifies how an animal coordinates its limbs during locomotion, providing objective measures of motor function in neuroscience research. During walking, researchers record and analyze variables such as step sequence, stride length, stance and swing duration, cadence, and interlimb coordination, often using video tracking or an instrumented walkway. These measurements reveal deficits in movement initiation, balance, coordination, or recovery after neural injury. Applied in studies of spinal cord injury, neurodegenerative disease, and motor-system development, stepping analysis helps evaluate disease progression and the effects of rehabilitation, pharmacological treatments, or other experimental interventions.

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JoVE Journal - Immunology and Infection
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Here, we present a protocol to identify potential anti-tuberculosis drug candidates through phenotypic screening, encompassing the chemical synthesis of a 4-aminoquinoline compound, determination of its minimum inhibitory concentration against Mycobacterium tuberculosis, and assessment of its cytotoxicity in mammalian cell lines.

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JoVE Journal - Medicine

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Cited by 5 •

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Education

JoVE Core - Nursing

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2024

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2024

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Cited by 23 •

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Nanodiscs are small discoid particles that incorporate membrane proteins into a small patch of phospholipid bilayer. We provide a visual protocol that shows the step-by-step incorporation of the MalFGK2 transporter into a disc.

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