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September 11, 2014
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Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
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Juliano, C. E., Lin, H., Steele, R. E. Generation of Transgenic Hydra by Embryo Microinjection. J. Vis. Exp. (91), e51888, doi:10.3791/51888 (2014).
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