A new ex vivo preparation for imaging the mouse spinal cord. This protocol allows for two-photon imaging of live cellular interactions throughout the spinal cord.
Weinger, J. G., Greenberg, M. L., Matheu, M. P., Parker, I., Walsh, C. M., Lane, T. E., Cahalan, M. D. Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord. J. Vis. Exp. (96), e52580, doi:10.3791/52580 (2015).