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June 23, 2016
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Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.
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Charuvi, D., Nevo, R., Kaplan-Ashiri, I., Shimoni, E., Reich, Z. Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy. J. Vis. Exp. (112), e54066, doi:10.3791/54066 (2016).
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