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June 20, 2016
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Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.
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Kowalko, J. E., Ma, L., Jeffery, W. R. Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs). J. Vis. Exp. (112), e54113, doi:10.3791/54113 (2016).
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