Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.
Scott, G. J., Gruzdev, A., Hagler, T. B., Ray, M. K. Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates. J. Vis. Exp. (135), e56955, doi:10.3791/56955 (2018).