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June 08, 2021
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Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.
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Chaihu, L., Yao, X., Xu, X., Zhu, Z., Chen, D. D. Y., Wang, G. Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry. J. Vis. Exp. (172), e62672, doi:10.3791/62672 (2021).
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