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September 20, 2021
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Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.
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Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K. M. Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing. J. Vis. Exp. (175), e62901, doi:10.3791/62901 (2021).
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