We describe a protocol for xenotransplantation into the yolk of transparent zebrafish embryos that is optimized by a simple, rapid staging method. Post-injection analyses include survival and assessing the disease burden of xenotransplanted cells by flow cytometry.
Verma, M., Rhodes, M., Shinton, S., Wiest, D. L. A Simple, Rapid, and Effective Method for Tumor Xenotransplantation Analysis in Transparent Zebrafish Embryos. J. Vis. Exp. (209), e66164, doi:10.3791/66164 (2024).