Dirk Grimm Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty University of Heidelberg Biography Publications Institution JoVE Articles Dirk Grimm has not added a biography. If you are Dirk Grimm and would like to personalize this page please email our Author Liaison for assistance. Publications Determination of AAV Properties by Single Amino Acids: Go(o)d is in the Details Molecular Therapy. Methods & Clinical Development. Dec, 2022 | Pubmed ID: 36212908 Semirational Bioengineering of AAV Vectors with Increased Potency and Specificity for Systemic Gene Therapy of Muscle Disorders Science Advances. 09, 2022 | Pubmed ID: 36129972 In Vivo Adenine Base Editing Reverts C282Y and Improves Iron Metabolism in Hemochromatosis Mice Nature Communications. 09, 2022 | Pubmed ID: 36064805 Boosters for Adeno-associated Virus Vector (AAV) (r)evolution Cytotherapy. Aug, 2022 | Pubmed ID: 35999132 Fantastic AAV Gene Therapy Vectors and How to Find Them-Random Diversification, Rational Design and Machine Learning Pathogens (Basel, Switzerland). Jul, 2022 | Pubmed ID: 35890005 Intranasal Application of Adeno-associated Viruses: a Systematic Review Translational Research : the Journal of Laboratory and Clinical Medicine. Oct, 2022 | Pubmed ID: 35597541 Identification of Adeno-associated Virus Variants for Gene Transfer into Human Neural Cell Types by Parallel Capsid Screening Scientific Reports. 05, 2022 | Pubmed ID: 35589936 Right on Target: The Next Class of Efficient, Safe, and Specific RNAi Triggers Molecular Therapy. Nucleic Acids. Jun, 2022 | Pubmed ID: 35505965 Natural Killer Cells Act As an Extrinsic Barrier for in Vivo Reprogramming Development (Cambridge, England). 04, 2022 | Pubmed ID: 35420133 Ex vivo and In vivo Suppression of SARS-CoV-2 with Combinatorial AAV/RNAi Expression Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. 05, 2022 | Pubmed ID: 35038579 An RNA Interference/Adeno-Associated Virus Vector-Based Combinatorial Gene Therapy Approach Against Hepatitis E Virus Hepatology Communications. 04, 2022 | Pubmed ID: 34719133 Breaking the Sound Barrier: Towards Next-generation AAV Vectors for Gene Therapy of Hearing Disorders Hearing Research. 01, 2022 | Pubmed ID: 33268240 Cas9-specific Immune Responses Compromise Local and Systemic AAV CRISPR Therapy in Multiple Dystrophic Canine Models Nature Communications. 11, 2021 | Pubmed ID: 34819506 Adeno-Associated Viruses (AAV) and Host Immunity - A Race Between the Hare and the Hedgehog Frontiers in Immunology. 2021 | Pubmed ID: 34777364 Lentiviral and Adeno-associated Vectors Efficiently Transduce Mouse T Lymphocytes when Targeted to Murine CD8 Molecular Therapy. Methods & Clinical Development. Dec, 2021 | Pubmed ID: 34729380 Neutralizing Antibody Evasion and Transduction with Purified Extracellular Vesicle-Enveloped Adeno-Associated Virus Vectors Human Gene Therapy. 12, 2021 | Pubmed ID: 34445894 The Angiopoietin-Tie2 Pathway Regulates Purkinje Cell Dendritic Morphogenesis in a Cell-autonomous Manner Cell Reports. 08, 2021 | Pubmed ID: 34407407 Membrane-destabilizing Ionizable Phospholipids: Novel Components for Organ-selective MRNA Delivery and CRISPR-Cas Gene Editing Signal Transduction and Targeted Therapy. 05, 2021 | Pubmed ID: 34035211 A Versatile In Vivo System to Study Myc in Cell Reprogramming Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 34019296 Best of Most Possible Worlds: Hybrid Gene Therapy Vectors Based on Parvoviruses and Heterologous Viruses Molecular Therapy : the Journal of the American Society of Gene Therapy. 12, 2021 | Pubmed ID: 33831556 Characterization of the GBoV1 Capsid and Its Antibody Interactions Viruses. 02, 2021 | Pubmed ID: 33672786 A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species Via Fluorescence-activated Cell Sorting Molecular Therapy. Methods & Clinical Development. Mar, 2021 | Pubmed ID: 33665228 'Hit and Run' Therapy Averts Macular Degeneration Nature Biomedical Engineering. 02, 2021 | Pubmed ID: 33580229 Optogenetic Control of Neisseria Meningitidis Cas9 Genome Editing Using an Engineered, Light-switchable Anti-CRISPR Protein Nucleic Acids Research. 03, 2021 | Pubmed ID: 33330940 Production, Processing, and Characterization of Synthetic AAV Gene Therapy Vectors Biotechnology Journal. Jan, 2021 | Pubmed ID: 32975881 Identification of a Myotropic AAV by Massively Parallel in Vivo Evaluation of Barcoded Capsid Variants Nature Communications. 10, 2020 | Pubmed ID: 33116134 Lab-Scale Production of Recombinant Adeno-Associated Viruses (AAV) for Expression of Optogenetic Elements Methods in Molecular Biology (Clifton, N.J.). 2020 | Pubmed ID: 32651911 Computational Design of Anti-CRISPR Proteins with Improved Inhibition Potency Nature Chemical Biology. 07, 2020 | Pubmed ID: 32284602 Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity Journal of Virology. 05, 2020 | Pubmed ID: 32213611 Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening Molecular Therapy : the Journal of the American Society of Gene Therapy. 04, 2020 | Pubmed ID: 32105604 Ex Vivo/In vivo Gene Editing in Hepatocytes Using "All-in-One" CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template IScience. Jan, 2020 | Pubmed ID: 31887661 Distinct Transduction of Muscle Tissue in Mice After Systemic Delivery of AAVpo1 Vectors Gene Therapy. 04, 2020 | Pubmed ID: 31624368 Severe Human Bocavirus 1 Respiratory Tract Infection in an Immunodeficient Child With Fatal Outcome The Pediatric Infectious Disease Journal. 09, 2019 | Pubmed ID: 31033910 Cell-specific CRISPR-Cas9 Activation by MicroRNA-dependent Expression of Anti-CRISPR Proteins Nucleic Acids Research. 07, 2019 | Pubmed ID: 30982889 Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30912043 Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses Molecular Therapy. Methods & Clinical Development. Mar, 2019 | Pubmed ID: 30766894 A Robust and All-Inclusive Pipeline for Shuffling of Adeno-Associated Viruses ACS Synthetic Biology. 01, 2019 | Pubmed ID: 30513195 Impact of the Assembly-Activating Protein on Molecular Evolution of Synthetic Adeno-Associated Virus Capsids Human Gene Therapy. 01, 2019 | Pubmed ID: 29978729 Engineered Anti-CRISPR Proteins for Optogenetic Control of CRISPR-Cas9 Nature Methods. 11, 2018 | Pubmed ID: 30377362 AAVvector-mediated in Vivo Reprogramming into Pluripotency Nature Communications. 07, 2018 | Pubmed ID: 29985406 High-Throughput Dissection of AAV-Host Interactions: The Fast and the Curious Journal of Molecular Biology. 08, 2018 | Pubmed ID: 29782834 Split Cas9, Not Hairs - Advancing the Therapeutic Index of CRISPR Technology Biotechnology Journal. Sep, 2018 | Pubmed ID: 29316283 Regulation of Adult CNS Axonal Regeneration by the Post-transcriptional Regulator Cpeb1 Frontiers in Molecular Neuroscience. 2017 | Pubmed ID: 29379413 Small But Increasingly Mighty: Latest Advances in AAV Vector Research, Design, and Evolution Human Gene Therapy. 11, 2017 | Pubmed ID: 28835125 Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells Journal of Virology. 10, 2017 | Pubmed ID: 28768875 Next-generation AAV Vectors for Clinical Use: an Ever-accelerating Race Virus Genes. Oct, 2017 | Pubmed ID: 28762205 Plasmodium Berghei EXP-1 Interacts with Host Apolipoprotein H During Plasmodium Liver-stage Development Proceedings of the National Academy of Sciences of the United States of America. Feb, 2017 | Pubmed ID: 28137845 TALEN/CRISPR-mediated Engineering of a Promoterless Anti-viral RNAi Hairpin into an Endogenous MiRNA Locus Nucleic Acids Research. 01, 2017 | Pubmed ID: 27614072 Blocking Sense-strand Activity Improves Potency, Safety and Specificity of Anti-hepatitis B Virus Short Hairpin RNA EMBO Molecular Medicine. 09, 2016 | Pubmed ID: 27473329 Death Receptor-based Enrichment of Cas9-expressing Cells BMC Biotechnology. Feb, 2016 | Pubmed ID: 26883910 Zinc Finger Nuclease-based Double-strand Breaks Attenuate Malaria Parasites and Reveal Rare Microhomology-mediated End Joining Genome Biology. Nov, 2015 | Pubmed ID: 26573820 AAV8-mediated in Vivo Overexpression of MiR-155 Enhances the Protective Capacity of Genetically Attenuated Malarial Parasites Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2014 | Pubmed ID: 25189739 表达式的哺乳动物阿耳戈英雄蛋白介导基因沉默的决定因素。 Nucleic Acids Research. Dec, 2011 | Pubmed ID: 22210886 成熟的肝细胞在小鼠肝内稳态和再生的命运跟踪。 The Journal of Clinical Investigation. Dec, 2011 | Pubmed ID: 22105172 剂量可以使毒药: 从 RNAi 表达所造成的不利体内毒性的教训。 Silence. 2011 | Pubmed ID: 22029761 去,还是不去,这就是问题-六个人思考如何地域流动性可能会影响你的事业和生活。 BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Oct, 2011 | Pubmed ID: 21858845 蜂窝网络不受控制的运行时: 全球时异常 RNAi 机制在人类病理学和治疗。 Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846572 细胞的 RNA 干扰机制。前言。 Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846566 小发夹 RNAs 的热力学稳定高度影响不同哺乳动物 Argonautes 加载的过程。 Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21576459 阿耳戈英雄蛋白质是 RNAi 药效、 毒性及成年小鼠肝脏中的持久性的关键决定因素。 The Journal of Clinical Investigation. Sep, 2010 | Pubmed ID: 20697157 六个 RNA 病毒和四十一个主机: 病毒性小 RNAs 和调制的脊椎动物和无脊椎动物系统中的小 RNA 曲目。 PLoS Pathogens. Feb, 2010 | Pubmed ID: 20169186 低级 ShRNA 细胞毒性可有助于成年小鼠 MYC 诱导肝细胞癌。 Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2010 | Pubmed ID: 19844192 小 RNAs 沉默: 先进。 Advanced Drug Delivery Reviews. Jul, 2009 | Pubmed ID: 19427885 从组织特异性 Pol II 启动子区 ShRNA 表达是有效和安全的 RNAi 治疗。 Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2008 | Pubmed ID: 18665161 沉默的肝脂肪酸转运蛋白 5 体内反转饮食诱导非酒精性脂肪性肝病,提高了高血糖。 The Journal of Biological Chemistry. Aug, 2008 | Pubmed ID: 18524776 通过 Multispecies 杂交和重定目标的腺相关病毒的体外和体内基因治疗矢量进化。 Journal of Virology. Jun, 2008 | Pubmed ID: 18400866 移植同种异体肝细胞诱导小鼠肝实质更换是促进骨髓移植和 CD4 细胞介导。 Hepatology (Baltimore, Md.). Feb, 2008 | Pubmed ID: 18220289 RNAi 治疗应用: 是 MRNA 针对最后准备就绪吗? The Journal of Clinical Investigation. Dec, 2007 | Pubmed ID: 18060021 Rnai 技术与基因治疗: 一种相互的吸引力。 Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program. 2007 | Pubmed ID: 18024667 进去得撞快速和稳定倒的内源性基因在视网膜色素上皮细胞中。 Human Gene Therapy. Oct, 2007 | Pubmed ID: 17892416 组合 RNAi: 与不断变化的目标赛跑的制胜策略? Molecular Therapy : the Journal of the American Society of Gene Therapy. May, 2007 | Pubmed ID: 17311009 双杂交屏幕标识组织蛋白酶 B 和 L 腺相关病毒 2 和 8 无涂层的考虑因素。 Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2007 | Pubmed ID: 17235311 37/67-Kilodalton 层粘连蛋白受体是一种受体的腺相关病毒血清型 8、 2、 3 和 9。 Journal of Virology. Oct, 2006 | Pubmed ID: 16973587 由于细胞 MicroRNA/短发夹 RNA 通路的荒野小鼠死亡。 Nature. May, 2006 | Pubmed ID: 16724069 改进的转录和调控的腺相关病毒载体靶向心脏基因转移。 Cardiovascular Research. Apr, 2006 | Pubmed ID: 16448634 肝与重组腺相关病毒转导是主要限制由衣壳血清型不是矢量基因型。 Journal of Virology. Jan, 2006 | Pubmed ID: 16352567 增加维护和持续存在的转基因表达盒从体内的质粒序列,切除。 Human Gene Therapy. May, 2005 | Pubmed ID: 15916481 短发夹 RNA 表达的腺相关病毒载体。 Methods in Enzymology. 2005 | Pubmed ID: 15644194 假型的腺相关病毒载体在肝脏基因治疗的临床前体内外评价。 Blood. Oct, 2003 | Pubmed ID: 12791653 腺相关病毒载体的血清型 1 到 6 的帮助器无病毒、 光学可控,和基于两个质粒的生产。 Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2003 | Pubmed ID: 12788658 基于腺相关病毒血清型基因转移载体的生产方法。 Methods (San Diego, Calif.). Oct, 2002 | Pubmed ID: 12413413 主细胞腺相关病毒 2 介导的基因转移和持续时间的记者基因表达的感病性不同。 Journal of Virological Methods. Sep, 2002 | Pubmed ID: 12270659 通过工程、条形码和筛选腺相关病毒 (AAV) 衣壳变体分离下一代基因治疗载体 Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengenharia 工程和演变的合成腺相关病毒(AAV),基因治疗载体,通过DNA家族改组 Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Imunologia e Infecção
通过工程、条形码和筛选腺相关病毒 (AAV) 衣壳变体分离下一代基因治疗载体 Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengenharia
工程和演变的合成腺相关病毒(AAV),基因治疗载体,通过DNA家族改组 Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Imunologia e Infecção