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Biology

Science Experiments

Advanced Biology

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Cell Biology

Tracking Internalized Membrane Proteins

Description

Cell-surface biotinylation assay is a method for tracking membrane proteins that move inside a cell through endocytosis. Endocytosis is the process cells use to take proteins from the membrane into the cytoplasm. Once inside, those proteins may be broken down or returned to the membrane by recycling.

Transcript

A rotulagem de proteínas de superfície celular com biotina apresenta uma poderosa ferramenta para estudar as vias de transporte celular envolvidas na regulação de proteínas de membrana. Uma célula mantém um conjunto bem regulado de proteínas na superfície, para que possa receber e responder a informações extracelulares através...

Tags

Endocytosis AssaySulfo NHS SS BiotinStreptavidin BeadsL Glutathione CleavageWestern BlottingProtein InternalizationMembrane Protein RecyclingDAT InternalizationCFTR Recycling

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