Tumor Cell Purification
Tumor Cell Purification - Related Videos
10:44
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
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Cited by 153 •
2011
Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
14:26
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
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Cited by 59 •
2016
This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.
08:16
Plasmid Purification
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2023
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.
03:22
Production and Purification of Recombinant Antibodies from Mammalian Cells
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2025
The video explains the process of producing antibodies in mammalian host cells, from gene expression to secretion. It involves transcription, translation, signal recognition, correct folding, and eventual release into the extracellular medium via secretory vesicles.
FAQs
The outcome depends on which distinguishing property best separates malignant cells from the surrounding sample. Surface-marker approaches target differences in cell-surface molecules, whereas density-, size-, or other physical-property-based methods exploit broader characteristics. Choosing an appropriate principle affects how effectively stromal, immune, and normal cells are reduced and how accurately the resulting population represents the tumor.
Surface markers provide a molecular basis for distinguishing tumor cells from other cell types in a heterogeneous specimen. Magnetic bead separation and flow cytometry can use these differences to enrich or isolate selected populations. Their value in cancer research is that reducing nonmalignant-cell contamination can improve interpretation of molecular, genomic, and transcriptomic measurements.
Purification can clarify which molecular or functional signals originate from malignant cells rather than from immune, stromal, or other normal cells. This distinction is important when tumors contain multiple cell populations with different properties. By producing a more focused population, the process supports investigation of tumor heterogeneity while helping researchers interpret differences within the cancer-cell compartment.
The choice depends on the feature available for distinguishing tumor cells in the sample. Magnetic bead separation and flow cytometry are suited to surface-marker-based selection, while density-gradient centrifugation uses differences in density. Researchers also need to consider whether the study requires enriched cells for molecular analysis or viable cells for downstream drug-response testing.
A general workflow begins with a tumor, blood, or tissue sample containing malignant and nonmalignant cells. Researchers then select a separation strategy based on surface markers, density, size, or another physical property, followed by enrichment of the desired population. The resulting cells can be directed toward molecular profiling, genomic or transcriptomic studies, or drug-response testing.
It is especially useful when signals from stromal, immune, or other normal cells could obscure cancer-cell measurements. Purified populations support molecular profiling, genomic and transcriptomic studies, drug-response testing, and analysis of tumor heterogeneity. The approach can therefore make cancer experiments more focused and improve the reproducibility of results obtained from complex biological samples.
Viability matters when purified cells must remain suitable for downstream testing rather than only serving as a source of molecular information. In particular, viable populations can support drug-response experiments and other analyses that depend on functioning cells. Balancing effective removal of nonmalignant cells with preservation of viability helps align the purification outcome with the research objective.