Collagen Bioink
Collagen Bioink - Related Videos
08:34
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Cited by 59 •
2016
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
07:03
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Cited by 18 •
2014
This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.
05:17
An Improved Method for the Preparation of Type I Collagen From Skin
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Cited by 23 •
2014
Traditional procedures for the isolation of soluble type 1 collagen (COL1) require about 10 days from start to finish because of lengthy buffer incubations and laborious resuspensions of fibrils. Here, we describe a means to purify COL1 from small dermal biopsies in less than 3 hr.
01:11
Fibril-associated Collagen
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2023
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix. For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
FAQs
Collagen provides a matrix context that cells can interact with while embedded in a three-dimensional structure. This interaction helps researchers examine how tumor cells respond to surrounding architecture and extracellular-matrix conditions, rather than observing cell behavior only in a simplified environment. The resulting model can support studies of tumor growth, invasion, and other cell behaviors.
These conditions control how the deposited collagen formulation undergoes gelation and becomes a stabilized structure. Temperature-, pH-, or crosslinking-dependent stabilization is important because the printed material must retain its three-dimensional form while remaining hydrated and compatible with encapsulated living cells. Selecting an appropriate stabilization approach therefore influences both model formation and its usefulness for cancer studies.
Three-dimensional architecture allows cancer models to reproduce aspects of tumor organization and matrix interaction that are difficult to represent in simpler in vitro arrangements. Cells experience a spatially structured, hydrated environment, enabling researchers to investigate tumor growth, invasion, and cell behavior in a context intended to be more physiologically relevant than conventional simplified models.
A typical workflow begins by preparing a collagen-based formulation that can contain living cells, followed by depositing it layer by layer through three-dimensional bioprinting. The printed construct is then stabilized through temperature-, pH-, or crosslinking-dependent gelation. This sequence produces a hydrated cellular structure suitable for examining cancer-related behavior in a controlled model.
Researchers may choose this approach when they need a three-dimensional tissue or disease model that includes both living cells and matrix interactions. Its cancer-research applications include examining tumor growth, invasion, and cell behavior, as well as creating platforms for drug screening. These uses are particularly relevant when physiological context is important for interpreting in vitro results.
These models can provide a structured setting for observing how cancer cells behave within a hydrated, three-dimensional matrix during drug studies. By incorporating tissue architecture and matrix interactions, they may help researchers assess responses in a setting with greater physiological relevance than simpler in vitro systems. The models therefore support comparative investigation of treatment effects alongside tumor-related behaviors.