Tubulin Markers
Tubulin Markers - Related Videos
02:40
Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects
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2025
Source: Piazza, V., et al., Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (2023)This video demonstrates the procedure for imaging microtubule abnormalities in a rat brain tissue slice using a two-photon excitation microscope. It employs second harmonic generation (SHG) signals to detect tubulin defects and reduced myelin production.
06:30
Optimizing Tubulin Yield from Porcine Brain Tissue
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2024
This protocol describes a technique for the high-yield isolation of tubulin from the porcine brain optimized for small-scale instrumentation. The isolation procedures are complemented by procedures for determining tubulin polymerization activity in vitro using co-sedimentation assays and transmission electron microscopy.
08:07
Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
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2023
In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.
09:38
Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers in vivo
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2026
Multiplexed immunofluorescence enables sequential labeling of multiple antibody markers within a single tissue section to support spatial analysis of tissue architecture and cell populations. Here, a workflow for multiplex detection of senescence-associated, structural, and immune markers is presented, including manual and automated slide preparation, iterative imaging, and single-cell analysis using digital pathology software.
07:54
Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
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Cited by 6 •
2020
This protocol describes tubulin purification from small/medium-scale sources such as cultured cells or single mouse brains, using polymerization and depolymerization cycles. The purified tubulin is enriched in specific isotypes or has specific posttranslational modifications and can be used in in vitro reconstitution assays to study microtubule dynamics and interactions.
FAQs
Their binding pattern provides spatial information about tubulin and the microtubule network. Markers can be selected to recognize particular tubulin isoforms or associated structures, allowing researchers to compare where these features occur and how their organization changes. In developing cells, this helps relate cytoskeletal arrangement to changing cellular architecture.
Changes in marker distribution or intensity can indicate differences in microtubule organization, apparent abundance, or architecture between cells, tissues, or developmental stages. These comparisons make it possible to examine cytoskeletal remodeling rather than viewing development only through overall tissue shape. The resulting patterns provide structural evidence for changes accompanying cell behavior.
Microtubule organization connects intracellular structure with processes such as division, migration, polarization, and neuronal process formation. When microtubule dynamics are disrupted, marker-based imaging can reveal associated changes in cellular architecture. This supports analysis of how altered cytoskeletal structure may contribute to developmental defects and abnormal tissue patterning.
Antibody-based markers identify tubulin through specific binding, whereas fluorescent tags provide a signal that can be visualized in microscopy. Both strategies can expose microtubule organization in cells and tissues, but the choice determines how tubulin features are detected and displayed. Their shared value is the conversion of molecular localization into interpretable structural patterns.
A typical approach applies a tubulin-directed marker to cells or tissues and then examines the resulting signal with microscopy, commonly immunofluorescence microscopy when antibodies are used. Researchers compare the labeled structures across developmental stages, regions, or experimental conditions. This workflow produces spatial evidence about microtubule arrangement and changes in cellular architecture.
They are particularly useful when a study needs to connect cytoskeletal structure with developmental behavior. Imaging can track microtubule patterns during cell division, migration, polarization, or neuronal process formation. The same strategy also supports studies of tissue patterning and cytoskeletal remodeling, making it relevant across cellular and tissue-level developmental analysis.
Tubulin marker imaging can reveal how microtubule organization, abundance, and cellular distribution differ among developmental states or experimental conditions. These observations help investigators evaluate changes in cell shape, tissue architecture, and neuronal process formation. In studies of disrupted microtubule dynamics, the patterns can also help associate structural defects with developmental outcomes.