Fluorescence-based Cell Counter
Fluorescence-based Cell Counter - Related Videos
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Cited by 18 •
2012
The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
07:31
Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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2024
A protocol is described for preparing a simple model ecosystem that recreates the methane-oxygen counter gradient found in the natural habitat of aerobic methane-oxidizing bacteria, enabling the study of their physiology in a spatially resolved context. Modifications to common biochemical assays for use with the agarose-based model ecosystem are also described.
05:02
Cell Electrofusion Visualized with Fluorescence Microscopy
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Cited by 11 •
2010
In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
FAQs
The instrument illuminates the prepared sample and records emitted light from fluorescently labeled cellular components. Image analysis then distinguishes those signals from background, allowing the system to identify cell-associated fluorescence rather than treating all detected light as cellular material. This separation supports more consistent estimates of cell number and concentration.
Viability dyes add a functional distinction to fluorescence measurements: they help separate intact cells from membrane-compromised cells. This distinction allows a count to be interpreted alongside viability rather than as a total signal alone. In genetics experiments, that context helps determine whether a manipulated cell population remains suitable for subsequent work.
Reliable cell concentration measurements make starting populations more comparable across experiments. When researchers use the measured values to establish cell seeding conditions, differences in later results are less likely to arise simply from unequal numbers of cells. This consistency is especially useful when assessing genetic manipulations or comparing experimental samples.
Researchers prepare a cell sample containing the relevant fluorescent labeling and, when viability is needed, a viability dye. The instrument illuminates the sample, captures emitted light, and applies image analysis. The resulting measurements can then be used to evaluate cell number, concentration, and viability for the experimental sample.
By measuring cell number and viability after a transfection or gene-editing experiment, researchers can assess the condition of the resulting cell population and compare samples more consistently. These measurements provide population-level context for interpreting genetic manipulation outcomes, especially before molecular analyses or additional experimental steps.
Before downstream molecular analysis, measurements of cell number, concentration, and viability give researchers a quantitative basis for preparing comparable samples. This information helps identify differences in population size or condition before molecular results are interpreted. As a result, researchers can better distinguish effects associated with genetic treatment from differences in the starting cell population.
Changes in cell number can indicate that a genetic manipulation has altered the cell population, while viability measurements show whether cells remain intact or membrane-compromised. The counter does not identify the molecular cause of a change by itself, but it supplies quantitative population information that helps researchers interpret transfection or gene-editing experiments.