Lymphocyte Metabolism
Lymphocyte Metabolism - Related Videos
11:29
HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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Cited by 5 •
2016
The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.
08:40
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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Cited by 38 •
2016
The study of metabolism is becoming increasingly relevant to immunological research. Here, we present an optimized method for measuring glycolysis and mitochondrial respiration in mouse splenocytes, and T and B lymphocytes.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
FAQs
Resting lymphocytes primarily rely on oxidative phosphorylation, but antigen recognition increases nutrient uptake and activates glycolysis, glutaminolysis, and biosynthetic pathways. This shift supplies both energy and building materials for clonal expansion. It also supports the production of cytokines, antibodies, or cytotoxic molecules needed for an effective immune response.
The mTOR pathway helps connect antigen-recognition signals with metabolic reprogramming. Through this signaling context, lymphocytes increase nutrient uptake and activate glycolytic, glutaminolytic, and biosynthetic activities. These coordinated changes allow cells to support rapid proliferation and effector functions rather than maintaining the metabolic pattern associated with a resting state.
Distinct metabolic programs help establish memory and regulate immune persistence after an immune response. This means lymphocyte metabolism contributes not only to immediate expansion and effector activity, but also to the longer-term maintenance of immune populations. Studying these programs can therefore clarify how immune responses persist beyond the initial antigen encounter.
Pathogens and tissue environments can shape the metabolic conditions in which lymphocytes respond. Their influence may affect nutrient use, pathway activation, immune-cell persistence, and effector activity. In immunology and infection research, examining these influences helps explain why lymphocyte responses differ across infectious settings and tissues, even when immune activation occurs.
Metabolic profiling examines the biochemical state of lymphocytes in relation to activation, infection, tissue conditions, or therapeutic intervention. It can reveal how pathogens and surrounding environments reshape energy production, biosynthetic activity, and immune function. These observations may identify metabolic features associated with effective responses, impaired persistence, inflammation, or therapeutic opportunity.
Metabolic therapies become relevant when changing lymphocyte pathways could alter immune outcomes. Research may use metabolic differences to identify targets for treating infection, inflammation, or cancer. The therapeutic goal is not limited to increasing activity; pathway-focused interventions may also help understand or regulate clonal expansion, effector function, memory formation, and immune persistence.