Tail Skin Excision
Tail Skin Excision - Related Videos
06:48
Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
0 Views •
Cited by 10 •
2014
Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.
03:21
Murine Tail Lymphedema Model: An In Vivo Technique to Generate Sustained Lymphedema in Murine Tail
0 Views •
2025
This video describes an in vivo technique to generate murine tail lymphedema, an abnormal fluid accumulation in the interstitial tissue space leading to swelling in the tail. The model is applied to study mechanisms and therapy for lymphedema.
10:17
The Tail Suspension Test
0 Views •
Cited by 534 •
2012
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
01:54
Base Excision Repair
0 Views •
2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
04:23
Frozen Skin Tissue Block Preparation: A Protocol to Preserve Cutaneous Melanoma Samples from Murine Dorsal Skin
0 Views •
2023
This video describes the detailed protocol for isolating melanoma-containing murine dorsal skin, followed by cryo-embedding to prepare a frozen skin tissue block. The cryo-embedded tissue block can be sectioned and microscopically examined to study melanoma progression.
FAQs
Using a defined tail location reduces variation caused by differences in sampled anatomy. Comparable tissue sections can then be processed across experimental groups using the same downstream analysis, making differences in leukocyte infiltration, inflammatory responses, tissue damage, or microbial burden easier to attribute to the experimental condition rather than inconsistent sampling.
The collected tissue can retain cellular and structural features needed for several complementary readouts. Tissue dissociation supports immune-cell recovery, while histology examines architecture and damage. The same sampling strategy can also support pathogen detection, allowing investigators to connect tissue organization, local immune activity, and evidence of infection within the sampled site.
Local tissue measurements show how host defense develops at the site of injury, inflammation, or infection, rather than only revealing changes elsewhere in the organism. Comparing tail-skin findings with broader experimental observations can help distinguish localized leukocyte recruitment or tissue damage from responses that reflect a more generalized disease or immune process.
Tail skin provides material for separate but related assessments of inflammatory response and microbial burden. Increased leukocyte infiltration or tissue damage indicates local immune and structural changes, whereas pathogen detection provides evidence related to infection. Evaluating these outcomes together can clarify whether tissue pathology accompanies detectable microbial presence or reflects another experimental effect.
The workflow begins by removing a defined section of tail skin, followed by directing the tissue to the analysis that matches the research question. It may be processed for dissociation and immune-cell recovery, prepared for histology, or examined for pathogen detection. Consistent site selection and tissue handling support meaningful comparisons among experimental groups.
Three supported downstream routes are tissue dissociation, histology, and pathogen detection. Dissociation enables recovery of cells for immunological assessment, histology preserves information about tissue structure and damage, and pathogen detection evaluates microbial burden. Selecting one or combining several routes allows the same sampling framework to address cellular, structural, and infectious outcomes.
This approach is useful when investigators need a standardized skin site for comparing local host responses across experimental groups. It can support studies of leukocyte infiltration, inflammation, tissue injury, and microbial burden. The method is especially relevant when the research objective requires linking a defined tissue response with broader mechanisms of host defense or disease.
Interpretation should consider the specific outcome measured and the consistency of the sampled site. Differences in recovered immune cells, tissue architecture, inflammatory changes, or pathogen detection can indicate altered local responses, but each reflects a different biological dimension. Comparing aligned readouts across groups helps determine whether changes concern immunity, tissue damage, infection, or a combination.