Store Operated Calcium Entry
Store Operated Calcium Entry - Related Videos
10:45
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Cited by 10 •
2017
Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
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Cited by 19 •
2012
The extent of store-operated Ca2+ entry (SOCE) can be monitored using fluorescent Ca2+ indicators. Mn2+ quenching of such indicators assays SOCE in cultured cells and skeletal muscle fibers. A technique allowing spatial and temporal resolution of SOCE by confocal imaging of mechanically skinned muscle fibers is also described.
06:34
Operation of a 25 KWth Calcium Looping Pilot-plant with High Oxygen Concentrations in the Calciner
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Cited by 7 •
2017
This manuscript describes a procedure for operating a calcium looping pilot-plant for post-combustion carbon capture with high oxygen concentrations in the calciner in order to reduce or eliminate the flue gas recycle.
09:17
Using a Virtual Store As a Research Tool to Investigate Consumer In-store Behavior
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Cited by 10 •
2017
This paper describes the use of a desktop virtual store to create virtual shopping environments to investigate in-store consumer behavior. A description of the protocol to build and run experiments, example results from an experiment concerning store layout, and important considerations when conducting virtual store experiments are presented.
01:12
Energy Stored in a Capacitor
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2023
When an archer pulls the string in a bow, he saves the work done in the form of elastic potential energy. When he releases the string, the potential energy is released as kinetic energy of the arrow. A capacitor works on the same principle in which the work done is saved as electric potential energy. The potential energy (UC) could be calculated by measuring the work done (W) to charge the capacitor. Let us consider the case of a parallel plate capacitor. When the capacitor is connected to a...
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The sequence begins when endoplasmic reticulum calcium stores decline. STIM1 detects this change and undergoes a conformational shift, then clusters close to the plasma membrane. This positioning enables interaction with Orai1 channels, whose opening permits extracellular calcium to enter. The ordered sequence connects internal store status to plasma-membrane calcium influx and restores calcium availability for continued signaling.
STIM1 acts as the store-status sensor, while Orai1 provides the entry route for calcium. Their division of labor means depletion is detected inside the cell, but replenishment occurs through calcium influx from outside. This arrangement links endoplasmic reticulum calcium balance with broader calcium signals that regulate neuronal activity and other calcium-dependent cellular processes.
In neurons, calcium signals associated with Store Operated Calcium Entry can influence excitability, the tendency of a neuron to respond electrically, as well as gene expression and synaptic function. The pathway also participates in calcium-dependent survival pathways, extending its relevance beyond short-term signaling. Altered entry could therefore affect immediate neuronal responses and longer-term cellular adaptation.
Calcium release from the endoplasmic reticulum reduces internal stores, whereas SOCE is engaged after depletion and brings extracellular calcium inward through Orai1. The two processes therefore have complementary directions and roles: one changes store content by releasing calcium, while the other helps replenish it. This distinction is useful when interpreting calcium signaling in neuronal cells.
A focused investigation can follow the pathway across three linked levels: store status, molecular response, and neuronal consequence. Researchers can relate calcium-store depletion to STIM1 activation and clustering, then examine Orai1 channel involvement and downstream effects on excitability, gene expression, synaptic function, or survival pathways. This organization separates the initiating signal from its neuronal outcomes.
The main outcomes include calcium balance, neuronal excitability, gene expression, synaptic function, and calcium-dependent survival. These endpoints span cellular maintenance, signaling, communication, and viability, allowing studies to determine whether a change in SOCE has localized or broad consequences. In neuroscience, this range connects molecular calcium regulation with both cellular physiology and neural function.
Its relevance comes from the pathway's position between calcium balance and neuronal signaling. Studying STIM1, Orai1, and their downstream neuronal effects may help clarify mechanisms associated with neurodevelopmental disorders, neurodegeneration, and altered circuit function. The same framework connects a molecular calcium-regulation event with broader questions about how neuronal systems develop, persist, or become functionally altered.