Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Purification and Visualization of Influenza A…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biology
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Studying DNA Looping by Single-Molecule FRET
Journal (Biology)
Studying DNA Looping by Single-Molecule FRET
Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
Journal (Biology)
Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
 

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Article DOI: 10.3791/1105-v • 09:36 min • February 9th, 2009
February 9th, 2009 •
1, 1, 1
1Department of Zoology, University of British Columbia - UBC

Capítulos

Resumo
Automatic Translation

The genome of the influenza A virus consists of eight separate complexes of RNA and proteins, termed viral ribonucleoprotein complexes (vRNPs). This paper describes the glycerol gradient purification and transmission electron microscopy visualization of influenza A vRNPs.

Tags

Purification Visualization Influenza A Viral Ribonucleoprotein Complexes VRNPs Viral Envelope Nuclear Import Replication Isolated VRNPs Procedure Disruption Detergents Glycerol Gradient Velocity Sedimentation SDS-PAGE Coomassie Blue Staining Peak Fractions Concentration Centrifugation Integrity Verification Transmission Electron Microscopy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter