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Een high-throughput methode om wereldwijd de studie van de morfologie organel in S. cerevisiae
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Een high-throughput methode om wereldwijd de studie van de morfologie organel in S. cerevisiae

Article DOI: 10.3791/1224-v • 07:29 min • March 2nd, 2009
March 2nd, 2009 •
1, 1, 1
1Department of Cellular and Physiological Sciences, University of British Columbia - UBC

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GFP-fusie-eiwitten worden op grote schaal gebruikt om de organellen visualiseren door confocale microscopie. Echter, screening voor mutaties die de morfologie van organellen invloed vereist in het algemeen individueel mutagenese en is tijdrovend. Hier tonen we een methode om simultaan te nemen organel-GFP markers in bijna 5.000 niet-essentiële genen in gist.

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Microbiologie High throughput confocale microscopie Acp1 mitochondria endoplasmatisch reticulum Saccharomyces cerevisiae
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