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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Article DOI: 10.3791/2189-v • 15:28 min • October 1st, 2010
October 1st, 2010 •
1, 1, 1
1Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies

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We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.

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Mutagenesis Functional Analysis Ion Channels Heterologously Expressed Mammalian Cells Point Mutation GIRK Channels G Protein-gated Inwardly Rectifying Potassium Channels Excitability Of Neurons G Protein-coupled Receptors Muscarinic Receptor Alcohol Activation Amino Acid Mutation CDNA Sequencing Transfection HEK293T Cells Patch-clamp Electrophysiology Potassium Currents L257W Mutation
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