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Capítulos
- 00:05Título
- 01:46Cell Lines, Vector Construction, and Lentiviral Production
- 03:19Production of Head and Neck Cell Lines with Stable LifeAct-mCherry Expression
- 04:30Orthotopic Tumor Xenograft Formation
- 05:56Preparation of Mouse Tongues for Ex-vivo Imaging
- 06:27Imaging of Tongue Tumors with Two-photon Microscopy and Image Analysis
- 09:55Representative Tumor Images
- 11:04Conclusion
A comprehensive overview of the techniques involved in generating a mouse model of oral cancer and quantitative monitoring of tumor invasion within the tongue through multi-photon microscopy of labeled cells is presented. This system can serve as a useful platform for the molecular assessment and drug efficacy of anti-invasive compounds.
Tags
Multi-photon ImagingTumor Cell InvasionOrthotopic Mouse ModelOral Squamous Cell CarcinomaHead And Neck CancerMetastatic RiskPatient Management StrategiesAnti-tumor TherapeuticsDisseminated Tumor CellsConventional HistologyIn Vivo Bioluminescent MethodsEarly Tumor Cell InvasionThree Dimensions VisualizationSquamous Cell Carcinoma Of The Tongue (SCOT)Two-photon ImagingLingual Tumor SpreadOSC-19 Head And Neck Tumor Cell LineF-actin Binding Peptide LifeActMCherry Fluorescent Protein
