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Journal / Neuroscience / 电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚…
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JoVE Journal Neuroscience
电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚
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Journal (Neuroscience)
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电穿孔轴突轨迹跟踪和突触目标的后脑在鸡胚

Article DOI: 10.3791/50136-v • 10:04 min • May 29th, 2013
May 29th, 2013 •
1, 2, 2, 1
1Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, 2Department of Medical Neurobiology, The Hebrew University of Jerusalem

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神经网络是建立在胚胎大脑发育神经生物学的一个基本问题。在这里，我们结合电穿孔技术与新的遗传工具，如酶Cre / LOX-质粒和PiggyBac转介导的DNA换位系统在禽流后脑背中间贴上标签，并跟踪他们的轴突预测和突触在不同发育阶段的目标。

Tags

神经，第75期，神经生物学，发育生物学，细胞生物学，分子生物学，解剖学，生理学，遗传学，电穿孔，小鸡，后脑，轴突，Interneuron，DA1，座子，增强，突触，神经元轴突，GFP表达，
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