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Journal / Immunology and Infection / 四环素调节细胞株产生高滴度的慢病毒载体，专门针对树突状细胞…
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Immunology and Infection

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JoVE Journal Immunology and Infection
四环素调节细胞株产生高滴度的慢病毒载体，专门针对树突状细胞
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四环素调节细胞株产生高滴度的慢病毒载体，专门针对树突状细胞

Article DOI: 10.3791/50606-v • 16:04 min • June 19th, 2013
June 19th, 2013 •
1, 1, 1, 1
1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles

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这里，我们用逆转录病毒转导和concatemeric的转染建立的细胞系中能表达的慢病毒载体（LV）的组件在没有四环素。此LV编码绿色荧光蛋白是一种糖蛋白，SVGmu，这是具体的树突状细胞上的受体与假。

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免疫学杂志，76期，病毒学，遗传学，分子生物学，细胞生物学，生物工程，生物医学工程，医学，感染，药理学，慢病毒，癌症疫苗，疫苗，病毒样颗粒，免疫学，生命科学，微生物学，生物工程（一般），慢病毒载体的稳定细胞系，树突状细胞，疫苗，concatemeric转染，逆转录病毒，病毒，质粒，细胞培养
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