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An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
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An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

Article DOI: 10.3791/50688-v • 10:34 min • August 7th, 2013
August 7th, 2013 •
1, 1, 2, 1, 1
1Pharmacology and Toxicology, Virginia Commonwealth University, 2Physiology and Biophyics, Virginia Commonwealth University

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We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

Tags

In-vitro Preparation Isolated Enteric Neurons Glia Myenteric Plexus Adult Mouse Gastrointestinal Tract Motility Control Isolation And Culture Primary Cultures Neuronal And Glia Population Enzymatic Digestion Sterile Conditions Centrifugation Coverslips Neurite Outgrowth Pan-neuronal Markers Patch Clamp Studies GFAP Staining Network Formation Immunohistochemistry Electrophysiology Calcium Imaging Single-cell PCR Genetically Modified Animals
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